When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

In a recent study posted to the medRxiv* preprint server, researchers examined sample collection practices, epidemiological characteristics, and cycle threshold (Ct) values for polymerase chain ...

PCR purification is a critical stage in molecular biology workflows requiring high-quality DNA fragments for sequencing, cloning, or genotyping. Multiple timed steps make it challenging to increase ...

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR ...

While the Delta variant of Covid-19 continues to swirl, many travel companies are cautiously churning back to business, albeit with modified protocols. Cruises are no exception, and Viking enlisted ...

The discovery of polymerase chain reaction (or PCR) in the 1980s sparked a molecular biology revolution. The reaction enables copious amounts of a target DNA sequence to be synthesized from a small ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...